人多能干细胞（PBMC来源）
货号：907005-PM726,907005-PM849,907005-PM853,907005-PM342,907005-PM705,907005-PM696,907005-PM2,907005-PM17,907005-PM3
规格：5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells
价格：6000,6000,6000,6000,6000,6000,6000,6000,6000
产品类型：原代细胞
品牌：PBM
- 加入购物车 {"thumb":"\/data\/upload\/63413d56e26d7.jpg","photo":{"1":{"url":"\/data\/upload\/63413d4bce9aa.jpg","alt":"Logo"},"2":{"url":"\/data\/upload\/63462dc6a6ddb.jpg","alt":"907005-PM3"},"3":{"url":"\/data\/upload\/63462dc6e8c5a.jpg","alt":"907005-PM17"},"4":{"url":"\/data\/upload\/63462dc7326f8.jpg","alt":"907005-PM342"},"5":{"url":"\/data\/upload\/63462dc76bae5.jpg","alt":"907005-PM696"},"6":{"url":"\/data\/upload\/63462dc79bee5.jpg","alt":"907005-PM705"},"7":{"url":"\/data\/upload\/63462dc8174b0.jpg","alt":"907005-PM726"},"8":{"url":"\/data\/upload\/63462dc843149.jpg","alt":"907005-PM849"},"9":{"url":"\/data\/upload\/63462dc8a2e01.jpg","alt":"907005-PM853"}}}
产品介绍
Human iPSCs 是由外周血细胞通过非整合型质粒电转的方法诱导获得。Human iPSCs 在多潜能干细胞培养体系中可以长期稳定快速增殖，具有高度近似人ES细胞的克隆形态和基因表达，长期维持正常核型，并在体内外具有三胚层分化潜能。 | |||
产品数据 | |||
 | A：细胞形态观察. B: iPSC 表达多能性因子Sox2, OCT4, Nanog和 TRA-1-60，比例尺: 100μm。C: ICC检测结果。D: 在 NOD/SCID 小鼠中 iPSC 形成畸胎瘤。图为三个胚层（中胚层：骨；内胚层：腺体；外胚层：神经管）HE染色结果，比例尺：40μm。 | ||
| 注意：人胚胎干细胞（hES）或诱导多能干细胞(hiPS)在无滋养层细胞培养条件下培养换液时，请将新鲜的培养液沿培养容器壁缓慢添加，避免液体的冲击力将贴壁的细胞吹起脱离培养皿。 | |||
| 其它人iPSC | |||
| 货号 | 来源 | 建立方法 | HLA配型** |
| 907005-PM342 | PBMCs | Episomal | A*11：01：01 |
| 907005-PM849 | PBMCs | Episomal | A*24：02：01 |
| 907005-PM853 | PBMCs | Episomal | A*02：01：01 |
| 907005-PM3 | PBMCs | Episomal | A*11：01：01 |
| 907005-PM726 | PBMCs | Episomal * | A*11：01：01 |
| 907005-PM17 | PBMCs | Episomal | A*11：01：01 |
| 907005-PM2 | PBMCs | Episomal | A*03：02：01G |
| 907005-PM696 | PBMCs | Episomal * | A*11：02：01G |
| 907005-PM705 | PBMCs | Episomal | A*24：02：01 |
| 907005-T849 | T cell | Sendai virus | A*24：02：01 |
| 907005-T853 | T cell | Sendai virus | A*02：01：01 |
| 907005-T17 | T cell | Sendai virus | A*11：01：01 |
| 907005-CBP35 | CBMNC | Episomal | A*01：01：01 |
| 907005-CB52 | CB CD34 | Episomal | A*02：01：01 |
| 相关产品 | |||
· SepTube-50密度梯度离心管（#601015） | |||
| 参考文献 | |||
1. Wei Wen#, Jian-Ping Zhang#, Jing Xu#, Ruijun Jeanna Su, Amanda Neises, Guang-Zhen Ji, Weiping Yuan, Tao Cheng*, Xiao-Bing Zhang* (2016). Enhanced Generation of Integration- free iPSCs from Human Adult Peripheral Blood Mononuclear Cells with an Optimal Combination of Episomal Vectors. Stem Cell Reports, 6(6):873-884.2. Wei Wen，Jian-Ping Zhang，Wanqiu Chen，Cameron Arakaki，Xiaolan Li， David Baylink， Gary D. Botimer，Jing Xu，Weiping Yuan，Tao Cheng*， Xiao-Bing Zhang* (2017). Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors. J. Vis. Exp. (119), e55091, doi:10.3791/55091.3. Wei Wen, Xinxin Cheng, Yawen Fu, Feiying Meng, Jian-Ping Zhang, Lu Zhang, Xiao-Lan Li, Zhixue Yang, Jing Xu, Feng Zhang, Gary D. Botimer, Weiping Yuan, Changkai Sun, Tao Cheng*, and Xiao-Bing Zhang* (2018). High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells, Stem Cell Reports, https://doi.org/10.1016/j.stemcr.2018.04.013 | |||
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