A:iPSC express pluripotency markers of OCT4, Sox2, SSEA-4 and TRA-1-60. Scale bars: 100μm. B: PCR-based detection of vector sequence (EBNA1 and OSW) was not found in the expanded iPSC after 5 passages. C: a representative karyotyping graph of an iPSC. D: iPSC form teratoma in NOD/SCID mice. H & E staining of representative teratoma from PB iPSC shows deribatives from three embryonic germ layers (mesoderm: bone, endoderm: glands, ectoderm: neurotubules), Scale bars: 40μm. |