货号:903105
规格: 5×10e5
价格:6000
产品类型:原代细胞
品牌:PBM
人诱导多能干(iPS)细胞系是通过将episomal质粒导入人骨髓单个核细胞(PBMC)使之表达多能性因子OCT4、SOX2、KLF4和c-MYC。细胞的选择以表性特征为标准,不通过荧光标记或药物筛选。在人ES细胞标准培养条件下进行培养时,人iPS细胞与ES细胞表型一致。该细胞同时也表达多能性因子TRA-1-60,SSEA-3和Oct4,并有很强的内源性碱性磷酸酶活性。 |
数据: |
A:iPSC express pluripotency markers of OCT4, Sox2, SSEA-4 and TRA-1-60. Scale bars: 100μm. B: PCR-based detection of vector sequence (EBNA1 and OSW) was not found in the expanded iPSC after 5 passages. C: a representative karyotyping graph of an iPSC. D: iPSC form teratoma in NOD/SCID mice. H & E staining of representative teratoma from PB iPSC shows deribatives from three embryonic germ layers (mesoderm: bone, endoderm: glands, ectoderm: neurotubules), Scale bars: 40μm.注意:人胚胎干细胞(hES)或诱导多能干细胞(hiPS)在无滋养层细胞培养条件下培养换液时,请将新鲜的培养液沿培养容器壁缓慢添加,避免液体的冲击力将贴壁的细胞吹起脱离培养皿。 |
相关产品: |
▪ iPS重编程培养基(货号#621050)▪ 多能干细胞培养基(货号#622050)▪ iPS早代细胞冻存液(货号#641010)▪ 0.25%胰酶(货号#607025)▪ 0.05%胰酶(货号#607005)▪ Y-27632小分子化合物(货号#215010) |
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